Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3073R-Cy3 |
| Product Name | Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HDAC4 around the phosphorylation site of Ser246 |
| Modification Site | Ser155 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9759 |
| Subcellular location | Nucleus |
| Synonyms | HDAC4Ser246; HDAC5Ser259; HDAC7Ser155; HD 4; HD4; HDAC 4; HDAC A; HDACA; Histone Deacetylase 4; Histone Deacetylase A; KIAA0288; EC 3.5.1.98; HA6116; p-HDAC4Ser246; p-HDAC5Ser259; p-HDAC7Ser155. |
| Background | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155) Polyclonal Antibody, Cy3 Conjugated (bs-3073R-Cy3) validated for, and at what dilution?
bs-3073R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155) Polyclonal Antibody, Cy3 Conjugated (bs-3073R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Horse, Chicken, Rabbit.
How should bs-3073R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-HDAC4 (Ser246) + HDAC5 (Ser259) + HDAC7 (Ser155) Polyclonal Antibody, Cy3 Conjugated (bs-3073R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human HDAC4 around the phosphorylation site of Ser246
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3073R-Cy3)?
Cy3 on bs-3073R-Cy3: excitation / emission 512,550nm/570,615nm.