phospho-MCSF Receptor (Tyr723) Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3077R-RBITC |
| Product Name | phospho-MCSF Receptor (Tyr723) Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Horse |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CSF1R around the phosphorylation site of tyrosine 723 |
| Modification Site | Tyr723 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1436 |
| Swiss Prot | P07333 |
| Subcellular location | Cell membrane |
| Synonyms | FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R; Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; Proto-oncogene c-Fms; CSF1R |
| Background | This protein tyrosine kinase transmembrane receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-MCSF Receptor (Tyr723) Polyclonal Antibody, RBITC Conjugated (bs-3077R-RBITC) validated for, and at what dilution?
bs-3077R-RBITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-MCSF Receptor (Tyr723) Polyclonal Antibody, RBITC Conjugated (bs-3077R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Horse.
How should bs-3077R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MCSF Receptor (Tyr723) Polyclonal Antibody, RBITC Conjugated (bs-3077R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human CSF1R around the phosphorylation site of tyrosine 723
What are the excitation and emission wavelengths of the RBITC conjugate (bs-3077R-RBITC)?
RBITC on bs-3077R-RBITC: excitation / emission 540nm/625nm.