phospho-MCSF Receptor (Tyr923) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3079R-APC-100uL |
| Product Name | phospho-MCSF Receptor (Tyr923) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CSF1R around the phosphorylation site of tyrosine 923 |
| Modification Site | Tyr923 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1436 |
| Swiss Prot | P07333 |
| Subcellular location | Cell membrane |
| Synonyms | FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R; Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; Proto-oncogene c-Fms; CSF1R |
| Background | Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is phospho-MCSF Receptor (Tyr923) Polyclonal Antibody, APC Conjugated (bs-3079R-APC) validated for, and at what dilution?
bs-3079R-APC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-MCSF Receptor (Tyr923) Polyclonal Antibody, APC Conjugated (bs-3079R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-3079R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-MCSF Receptor (Tyr923) Polyclonal Antibody, APC Conjugated (bs-3079R-APC)?
KLH conjugated synthetic phosphopeptide derived from human CSF1R around the phosphorylation site of tyrosine 923
What are the excitation and emission wavelengths of the APC conjugate (bs-3079R-APC)?
APC on bs-3079R-APC: excitation / emission 650nm/660nm.