phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3081R-Cy5 |
| Product Name | phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to Tyr231 in CaMK2B and CaMK2D and will react with these proteins when phosphorylated at this site. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CaMK2 alpha around the phosphorylation site of Tyr230 |
| Modification Site | Tyr230 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 815 |
| Swiss Prot | Q9UQM7 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CAMKA; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2A; KIAA0968 |
| Background | CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody, Cy5 Conjugated (bs-3081R-Cy5) validated for, and at what dilution?
bs-3081R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody, Cy5 Conjugated (bs-3081R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-3081R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody, Cy5 Conjugated (bs-3081R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human CaMK2 alpha around the phosphorylation site of Tyr230
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3081R-Cy5)?
Cy5 on bs-3081R-Cy5: excitation / emission 625,650nm/670nm.