Phospho-Beta catenin (Thr41 + Ser45) Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3088R-BF555 |
| Product Name | Phospho-Beta catenin (Thr41 + Ser45) Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phophorylation site is homologous to that of Thr41 + Ser45 in Mouse and Rat. |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Beta-Catenin around the phosphorylation site of Thr41/Ser45 |
| Modification Site | Thr41 + Ser45 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1499 |
| Swiss Prot | P35222 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane, Extracellular matrix |
| Synonyms | CTNNB; MRD19; armadillo; Catenin beta-1; Beta-catenin; CTNNB1; OK/SW-cl.35; PRO2286 |
| Background | Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Beta catenin (Thr41 + Ser45) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3088R-BF555) validated for, and at what dilution?
bs-3088R-BF555 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Beta catenin (Thr41 + Ser45) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3088R-BF555) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-3088R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Beta catenin (Thr41 + Ser45) Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-3088R-BF555)?
KLH conjugated synthetic phosphopeptide derived from human Beta-Catenin around the phosphorylation site of Thr41/Ser45
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-3088R-BF555)?
AbBy Fluor-555 on bs-3088R-BF555: excitation / emission 553nm/568nm.