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Phospho-cdc25A (Ser76) Polyclonal Antibody

Mouse Embryo lysates probed with Phospho-cdc25A (Ser76) Polyclonal Antibody, Unconjugated (bs-3094R) at 1:500 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.
Mouse Embryo lysates probed with Phospho-cdc25A (Ser76) Polyclonal Antibody, Unconjugated (bs-3094R) at 1:500 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.
Lane 1: Mouse Testis lysates; Lane 2: Human MCF-7 lysates probed with Phospho-cdc25A (Ser76) Polyclonal Antibody, Unconjugated (bs-3094R) at 1:500 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.
Lane 1: Mouse Testis lysates; Lane 2: Human MCF-7 lysates probed with Phospho-cdc25A (Ser76) Polyclonal Antibody, Unconjugated (bs-3094R) at 1:500 dilution and 4\u02daC overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37\u02daC.
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-cdc25A (Ser76) Antibody(bs-3094R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
HepG2 cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with Phospho-cdc25A (Ser76) Antibody(bs-3094R)at 1:50 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Horse
  • Rabbit
Overview
Catalog # bs-3094R
Product Name Phospho-cdc25A (Ser76) Polyclonal Antibody
Applications
WB ELISA
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human cdc25A around the phosphorylation site of Ser76 [MG(p-S)SE]
Modification Site Ser76
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 993
Subcellular location Cytoplasm, Nucleus
Synonyms cdc25ASer76; Cdc 25a; CDC25A2; Cell division cycle 25 homolog A S. pombe; Cell division cycle 25A; Cell division cycle 25A isoform a; Cell division cycle 25A isoform bv D9Ertd393ev Dual specicity phosphatase CDC25A; EC 3.1.3.48; M phase inducer phosphatase 1; MGC115549;
Background Cyclin dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of Wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1 S phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000

Frequently asked questions

Which applications is Phospho-cdc25A (Ser76) Polyclonal Antibody (bs-3094R) validated for, and at what dilution?

bs-3094R is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-cdc25A (Ser76) Polyclonal Antibody (bs-3094R) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Horse, Rabbit.

How should bs-3094R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-cdc25A (Ser76) Polyclonal Antibody (bs-3094R)?

KLH conjugated synthetic phosphopeptide derived from human cdc25A around the phosphorylation site of Ser76 [MG(p-S)SE]

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