Phospho-cdc25C (Ser216) Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3096R-BF647-100uL |
| Product Name | Phospho-cdc25C (Ser216) Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is found at Ser173 in Isoform 3 of this protein. |
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216 |
| Modification Site | Ser216 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 995 |
| Swiss Prot | P30307 |
| Subcellular location | Nucleus |
| Synonyms | CDC25; PPP1R60; M-phase inducer phosphatase 3; Dual specificity phosphatase Cdc25C; CDC25C |
| Background | Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-cdc25C (Ser216) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3096R-BF647) validated for, and at what dilution?
bs-3096R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3096R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-cdc25C (Ser216) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3096R-BF647)?
KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-3096R-BF647)?
AbBy Fluor-647 on bs-3096R-BF647: excitation / emission 650nm/665nm.