Phospho-Cofilin (Ser3) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3099R-100uL |
| Product Name | Phospho-Cofilin (Ser3) Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous in the listed cross reactive species at the specified location. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Sheep, Pig, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Cofilin around the phosphorylation site of Ser3 |
| Modification Site | Ser3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1072 |
| Swiss Prot | P23528 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CFL; cofilin; HEL-S-15; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin, non-muscle isoform; CFL1 |
| Background | Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Cofilin (Ser3) Polyclonal Antibody (bs-3099R) validated for, and at what dilution?
bs-3099R is validated for WB (1:300-5000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Cofilin (Ser3) Polyclonal Antibody (bs-3099R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Pig, Horse.
How should bs-3099R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Cofilin (Ser3) Polyclonal Antibody (bs-3099R)?
KLH conjugated synthetic phosphopeptide derived from human Cofilin around the phosphorylation site of Ser3