Phospho-DOK2 (Tyr299) Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3102R-BF594 |
| Product Name | Phospho-DOK2 (Tyr299) Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Dog, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human p56Dok2 around the phosphorylation site of Tyr299 |
| Modification Site | Tyr299 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9046 |
| Subcellular location | Cytoplasm |
| Synonyms | Docking protein 2 56kDa; Docking protein 2 antibody DOK 2; DOK R; Downstream of tyrosine kinase 2; p56dok 2; p56dok 2; p56DOK; p56dok2; DOK2; DOK2_HUMAN; Downstream of tyrosine kinase 2; OTTHUMP00000161710; OTTHUMP00000224923; p56dok 2; p56dok-2. |
| Background | The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-DOK2 (Tyr299) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-3102R-BF594) validated for, and at what dilution?
bs-3102R-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3102R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-DOK2 (Tyr299) Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-3102R-BF594)?
KLH conjugated synthetic phosphopeptide derived from human p56Dok2 around the phosphorylation site of Tyr299
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-3102R-BF594)?
AbBy Fluor-594 on bs-3102R-BF594: excitation / emission 590nm/617nm.