Phospho-DARPP32 (Thr34) Polyclonal Antibody, PerCP Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3118R-PerCP-100uL |
| Product Name | Phospho-DARPP32 (Thr34) Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous in the listed cross reactive species at the specified location. |
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human DARPP32 around the phosphorylation site of Thr34 |
| Modification Site | Thr34 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84152 |
| Swiss Prot | Q9UD71 |
| Subcellular location | Cytoplasm |
| Synonyms | DARPP32; DARPP-32; Protein phosphatase 1 regulatory subunit 1B; Dopamine- and cAMP-regulated neuronal phosphoprotein; PPP1R1B |
| Background | Inhibitor of protein-phosphatase 1. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-DARPP32 (Thr34) Polyclonal Antibody, PerCP Conjugated (bs-3118R-PerCP) validated for, and at what dilution?
bs-3118R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3118R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-DARPP32 (Thr34) Polyclonal Antibody, PerCP Conjugated (bs-3118R-PerCP)?
KLH conjugated synthetic phosphopeptide derived from human DARPP32 around the phosphorylation site of Thr34
What are the excitation and emission wavelengths of the PerCP conjugate (bs-3118R-PerCP)?
PerCP on bs-3118R-PerCP: excitation / emission 482nm/677nm.