phospho-CRK (Tyr221) Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3121R-BF647-100uL |
| Product Name | phospho-CRK (Tyr221) Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Others |
| Predicted Reactivity | Human, Mouse, Rat, Dog, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Crk p38 around the phosphorylation site of Tyr221 [GP(p-Y)AQ] |
| Modification Site | Tyr221 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1398 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Adapter molecule crk; Proto-oncogene c-Crk; p38; CRK |
| Background | The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CRK (Tyr221) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3121R-BF647) validated for, and at what dilution?
bs-3121R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3121R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CRK (Tyr221) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3121R-BF647)?
KLH conjugated synthetic phosphopeptide derived from human Crk p38 around the phosphorylation site of Tyr221 [GP(p-Y)AQ]
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-3121R-BF647)?
AbBy Fluor-647 on bs-3121R-BF647: excitation / emission 650nm/665nm.