Phospho-Cyclin B1 (Ser147) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3122R-Cy5.5-100uL |
| Product Name | Phospho-Cyclin B1 (Ser147) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser147 |
| Modification Site | Ser147 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 891 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Cyclin B1 phospho S147; Cyclin B1 Ser147; p-Cyclin B1 S147; p-Cyclin B1 Ser147; p-Cyclin B1 phospho S147; CCNB 1; CCNB; CCNB1; CCNB1_HUMAN; G2 mitotic specic cyclin B1; G2/mitotic-specic cyclin-B1. |
| Background | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Cyclin B1 (Ser147) Polyclonal Antibody, Cy5.5 Conjugated (BS-3122R-CY5-5) validated for, and at what dilution?
BS-3122R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Cyclin B1 (Ser147) Polyclonal Antibody, Cy5.5 Conjugated (BS-3122R-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, GuineaPig.
How should BS-3122R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Cyclin B1 (Ser147) Polyclonal Antibody, Cy5.5 Conjugated (BS-3122R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of Ser147
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-3122R-CY5-5)?
Cy5.5 on BS-3122R-CY5-5: excitation / emission 675nm/694nm.