phospho-HDAC8 (Ser39) Polyclonal Antibody, Cy5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3127R-Cy5-100uL |
| Product Name | phospho-HDAC8 (Ser39) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit, Guinea Pig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HDAC8 around the phosphorylation site of Ser39 |
| Modification Site | Ser39 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 55869 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HDAC8 Ser39; HD 8; HD8; HDAC 8; HDACL 1; HDACL1; Histone deacetylase 8; Histone deacetylase like 1; RPD 3; RPD3. |
| Background | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-HDAC8 (Ser39) Polyclonal Antibody, Cy5 Conjugated (bs-3127R-Cy5) validated for, and at what dilution?
bs-3127R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3127R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-HDAC8 (Ser39) Polyclonal Antibody, Cy5 Conjugated (bs-3127R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human HDAC8 around the phosphorylation site of Ser39
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3127R-Cy5)?
Cy5 on bs-3127R-Cy5: excitation / emission 625,650nm/670nm.