phospho-Ephrin B (Tyr324 + Tyr329) Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3129R-BF647 |
| Product Name | phospho-Ephrin B (Tyr324 + Tyr329) Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | These phosphorylation sites are homologous to Tyr323 + Tyr328 in Mouse. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr324/329 |
| Modification Site | Tyr324 + Tyr329 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1947 |
| Swiss Prot | P98172 |
| Subcellular location | Cell membrane |
| Synonyms | CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2; Ephrin-B1; EFL-3; ELK ligand; EPH-related receptor tyrosine kinase ligand 2; LERK-2; EFNB1 |
| Background | Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity). Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to the receptor tyrosine kinases EPHB3 (preferred), EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Ephrin B (Tyr324 + Tyr329) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3129R-BF647) validated for, and at what dilution?
bs-3129R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Ephrin B (Tyr324 + Tyr329) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3129R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-3129R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Ephrin B (Tyr324 + Tyr329) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3129R-BF647)?
KLH conjugated synthetic phosphopeptide derived from human Ephrin B around the phosphorylation site of Tyr324/329
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-3129R-BF647)?
AbBy Fluor-647 on bs-3129R-BF647: excitation / emission 650nm/665nm.