Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch
Help

Phospho-Ezrin (Thr567) Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-Ezrin (Thr567)) Polyclonal Antibody, Unconjugated (bs-3134R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-Ezrin (Thr567)) Polyclonal Antibody, Unconjugated (bs-3134R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-Ezrin (Thr567)) Polyclonal Antibody, Unconjugated (bs-3134R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Phospho-Ezrin (Thr567)) Polyclonal Antibody, Unconjugated (bs-3134R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
  • Guinea Pig
Overview
Catalog # bs-3134R
Product Name Phospho-Ezrin (Thr567) Polyclonal Antibody
Applications
WB IHC-P IHC-F IF
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, Guinea Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic phosphopeptide derived from human Ezrin around the phosphorylation site of Thr567
Modification Site Thr567
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7430
Subcellular location Cytoplasm, Cell membrane
Synonyms Ezrin phospho T567; Ezrin phospho Thr567; p-Ezrin phospho T567; CVIL; CVL; cytovillin 2 ; Cytovillin ; DKFZp762H157; FLJ26216; MGC1584; p81; VIL 2 ; VIL2; Villin 2; Villin2; EZRI_HUMAN.
Background The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Application Dilution
WB 1:300-5000
IHC-P ELISA1:500-1000
IHC-F
IF

Frequently asked questions

Which applications is Phospho-Ezrin (Thr567) Polyclonal Antibody (bs-3134R) validated for, and at what dilution?

bs-3134R is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-Ezrin (Thr567) Polyclonal Antibody (bs-3134R) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, Guinea Pig.

How should bs-3134R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-Ezrin (Thr567) Polyclonal Antibody (bs-3134R)?

KLH conjugated synthetic phosphopeptide derived from human Ezrin around the phosphorylation site of Thr567

Search our catalog of hundreds of thousands of products