Phospho-Ezrin (Thr567) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3134R-Cy3-100uL |
| Product Name | Phospho-Ezrin (Thr567) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, Guinea Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Ezrin around the phosphorylation site of Thr567 |
| Modification Site | Thr567 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7430 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Ezrin phospho T567; Ezrin phospho Thr567; p-Ezrin phospho T567; CVIL; CVL; cytovillin 2 ; Cytovillin ; DKFZp762H157; FLJ26216; MGC1584; p81; VIL 2 ; VIL2; Villin 2; Villin2; EZRI_HUMAN. |
| Background | The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is Phospho-Ezrin (Thr567) Polyclonal Antibody, Cy3 Conjugated (bs-3134R-Cy3) validated for, and at what dilution?
bs-3134R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Ezrin (Thr567) Polyclonal Antibody, Cy3 Conjugated (bs-3134R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit, Guinea Pig.
How should bs-3134R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Ezrin (Thr567) Polyclonal Antibody, Cy3 Conjugated (bs-3134R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human Ezrin around the phosphorylation site of Thr567
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3134R-Cy3)?
Cy3 on bs-3134R-Cy3: excitation / emission 512,550nm/570,615nm.