Phospho-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3137R-APC-Cy7-100uL |
| Product Name | Phospho-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human FOXO1 around the phosphorylation site of Thr24+Thr32 |
| Modification Site | Thr24+Thr32 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2308 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | FOXO4 phospho T32; p-FoxO1 Thr24/FoxO3a Thr32; FOXO3A phospho T32; p-FOXO3A phospho T32; Forkhead box protein O1; Afxh; AI876417; FKHR; Fkhr1; Foxo1a; Forkhead; FKH 1; FKH1; FKH1; FKHR; FKHR; Forkhead Drosophila homolog 1 rhabdomyosarcoma; Forkhead Drosophila homolog 1 rhabdomyosarcoma; Forkhead box O1; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; Forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOXO1; FOXO1; FOXO1_HUMAN; FOXO1A; forkhead box protein O3. |
| Background | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3137R-APC-Cy7) validated for, and at what dilution?
bs-3137R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3137R-APC-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Chicken, Rabbit.
How should bs-3137R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3137R-APC-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human FOXO1 around the phosphorylation site of Thr24+Thr32
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-3137R-APC-Cy7)?
APC-Cy7 on bs-3137R-APC-Cy7: excitation / emission 650nm/780nm.