Phospho-FoxO3a (Ser253) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3140R-FITC |
| Product Name | Phospho-FoxO3a (Ser253) Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to Ser252 in Mouse and Rat. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human FoxO3a around the phosphorylation site of Ser253 |
| Modification Site | Ser253 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2309 |
| Swiss Prot | O43524 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3 |
| Background | Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34C expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-FoxO3a (Ser253) Polyclonal Antibody, FITC Conjugated (bs-3140R-FITC) validated for, and at what dilution?
bs-3140R-FITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-FoxO3a (Ser253) Polyclonal Antibody, FITC Conjugated (bs-3140R-FITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken.
How should bs-3140R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-FoxO3a (Ser253) Polyclonal Antibody, FITC Conjugated (bs-3140R-FITC)?
KLH conjugated synthetic phosphopeptide derived from human FoxO3a around the phosphorylation site of Ser253
What are the excitation and emission wavelengths of the FITC conjugate (bs-3140R-FITC)?
FITC on bs-3140R-FITC: excitation / emission 494nm/518nm.