Phospho-FoxO1 (Ser256) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3142R-Cy5.5-100uL |
| Product Name | Phospho-FoxO1 (Ser256) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Rabbit |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human FOXO1 around the phosphorylation site of Ser256 [AA(p-S)MD] |
| Modification Site | Ser256 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2308 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | FOXO1A phospho S256; p-FOXO1A phospho S256; FKHRSer256; Forkhead box protein O1; Afxh; AI876417; FKHR; Fkhr1; Foxo1a; Forkhead; FKH 1; FKH1; FKH1; FKHR; FKHR; Forkhead Drosophila homolog 1 rhabdomyosarcoma; Forkhead Drosophila homolog 1 rhabdomyosarcoma; Forkhead box O1; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; Forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOXO1; FOXO1; FOXO1_HUMAN; FOXO1A. |
| Background | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-FoxO1 (Ser256) Polyclonal Antibody, Cy5.5 Conjugated (BS-3142R-CY5-5) validated for, and at what dilution?
BS-3142R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-FoxO1 (Ser256) Polyclonal Antibody, Cy5.5 Conjugated (BS-3142R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken.
How should BS-3142R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-FoxO1 (Ser256) Polyclonal Antibody, Cy5.5 Conjugated (BS-3142R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human FOXO1 around the phosphorylation site of Ser256 [AA(p-S)MD]
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-3142R-CY5-5)?
Cy5.5 on BS-3142R-CY5-5: excitation / emission 675nm/694nm.