phospho-FOXO4 (Ser197) Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3144R-PE-Cy5 |
| Product Name | phospho-FOXO4 (Ser197) Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human FOXO4 around the phosphorylation site of Ser197 |
| Modification Site | Ser197/Ser256 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4303 |
| Swiss Prot | Q9WVH3 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | FOXO4 phospho S197; p-FOXO4 phospho S197; AFXSer197; Fork head domain transcription factor AFX1; AFX; AFX1; Afxh; ALL1-fused gene from X chromosome; Fork head domain transcription factor AFX1; Forkhead box O4; Forkhead box protein O4; FOXO 4; Mixed lineage leukemia, translocated to, 7; MLLT7; Myeloid lymphoid or mixed lineage leukemia translocated to 7; Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7; Putative fork head domain transcription factor AFX1, FOXO1 Phospho-Ser256, Forkhead box protein O1 Phospho Ser256, FKHR Phospho-Ser256, FOXO1A Phospho-Ser256. |
| Background | These genes encode a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. Multiple transcript variants encoding different isoforms have been found for these genes. Both function as transcription factors involved in the regulation of the insulin signaling pathway. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is phospho-FOXO4 (Ser197) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3144R-PE-Cy5) validated for, and at what dilution?
bs-3144R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-FOXO4 (Ser197) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3144R-PE-Cy5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-3144R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-FOXO4 (Ser197) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3144R-PE-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human FOXO4 around the phosphorylation site of Ser197
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-3144R-PE-Cy5)?
PE-Cy5 on bs-3144R-PE-Cy5: excitation / emission 488nm/670nm.