Phospho-c-Fos (Thr325) Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3154R-BF647-100uL |
| Product Name | Phospho-c-Fos (Thr325) Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to Thr325 in Mouse and Rat. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Sheep, Pig, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325 |
| Modification Site | Thr325 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2353 |
| Swiss Prot | P01100 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | p55; AP-1; C-FOS; Proto-oncogene c-Fos; Cellular oncogene fos; G0/G1 switch regulatory protein 7; FOS; G0S7 |
| Background | Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-c-Fos (Thr325) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3154R-BF647) validated for, and at what dilution?
bs-3154R-BF647 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-c-Fos (Thr325) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3154R-BF647) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Sheep, Pig, Chicken.
How should bs-3154R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-c-Fos (Thr325) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-3154R-BF647)?
KLH conjugated synthetic phosphopeptide derived from human c-Fos around the phosphorylation site of Thr325
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-3154R-BF647)?
AbBy Fluor-647 on bs-3154R-BF647: excitation / emission 650nm/665nm.