Phospho-GCN2 (Thr899) Polyclonal Antibody

$Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GCN2 (Thr899) Polyclonal Antibody(bs-3155R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GCN2 (Thr899) Polyclonal Antibody(bs-3155R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GCN2 (Thr899) Polyclonal Antibody(bs-3155R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

WB
ELISA
FCM

Reactivity

Human
Mouse

Predicted Reactivity

Rat
Dog
Cow
Horse

Overview
Catalog #	bs-3155R
Product Name	Phospho-GCN2 (Thr899) Polyclonal Antibody
Applications	WB, ELISA, FCM
Specificity	This phosphorylation site is homologous to Thr898 in Mouse and Thr899 in Rat.
Reactivity	Human, Mouse
Predicted Reactivity	Rat, Dog, Cow, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899
Modification Site	Thr899
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	440275
Swiss Prot	Q9P2K8
Subcellular location	Cytoplasm
Synonyms	GCN2; PVOD2; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2-like protein; EIF2AK4; KIAA1338
Background	GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
FCM	1:20-100