Phospho-GCN2 (Thr899) Polyclonal Antibody, APC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3155R-APC |
| Product Name | Phospho-GCN2 (Thr899) Polyclonal Antibody, APC Conjugated |
| Applications |
WB
FCM
|
| Specificity | This phosphorylation site is homologous to Thr898 in Mouse and Thr899 in Rat. |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Horse |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899 |
| Modification Site | Thr899 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 440275 |
| Swiss Prot | Q9P2K8 |
| Subcellular location | Cytoplasm |
| Synonyms | GCN2; PVOD2; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2-like protein; EIF2AK4; KIAA1338 |
| Background | GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-GCN2 (Thr899) Polyclonal Antibody, APC Conjugated (bs-3155R-APC) validated for, and at what dilution?
bs-3155R-APC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-GCN2 (Thr899) Polyclonal Antibody, APC Conjugated (bs-3155R-APC) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Horse.
How should bs-3155R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-GCN2 (Thr899) Polyclonal Antibody, APC Conjugated (bs-3155R-APC)?
KLH conjugated synthetic phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899
What are the excitation and emission wavelengths of the APC conjugate (bs-3155R-APC)?
APC on bs-3155R-APC: excitation / emission 650nm/660nm.