phospho-GCN2 (Thr667) Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3156R-BF350-100uL |
| Product Name | phospho-GCN2 (Thr667) Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to Thr666 in Mouse and Thr667 in Rat. |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Cow, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human GCN2 around the phosphorylation site of Thr667 |
| Modification Site | Thr667 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 440275 |
| Swiss Prot | Q9P2K8 |
| Subcellular location | Cytoplasm |
| Synonyms | GCN2; PVOD2; Eukaryotic translation initiation factor 2-alpha kinase 4; GCN2-like protein; EIF2AK4; KIAA1338 |
| Background | GCN2 belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor 2 to downregulate protein synthesis in response to varied cellular stresses. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-GCN2 (Thr667) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-3156R-BF350) validated for, and at what dilution?
bs-3156R-BF350 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-GCN2 (Thr667) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-3156R-BF350) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Horse.
How should bs-3156R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-GCN2 (Thr667) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-3156R-BF350)?
KLH conjugated synthetic phosphopeptide derived from human GCN2 around the phosphorylation site of Thr667
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-3156R-BF350)?
AbBy Fluor-350 on bs-3156R-BF350: excitation / emission 346nm/442nm.