Phospho-GRB10 (Tyr67) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3157R |
| Product Name | Phospho-GRB10 (Tyr67) Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human GRB10 around the phosphorylation site of Tyr67 [SL(p-Y)SA] |
| Modification Site | Tyr67 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2887 |
| Subcellular location | Cytoplasm |
| Synonyms | GRB IR; grb-10; GRB10 adaptor protein; GRBIR; Growth factor receptor bound protein 10; Insulin receptor binding protein; Insulin receptor binding protein GRB IR; IRBP; MEG1; RSS. |
| Background | GRB 10 belongs to a small family of adapter proteins that interact with receptor tyrosine kinases and signaling molecules. In particular, GRB 10 interacts with insulin receptors and insulin-like growth-factor receptors. Alternatively spliced transcript variants encoding different isoforms have been identified: overexpression of some of them inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-GRB10 (Tyr67) Polyclonal Antibody (bs-3157R) validated for, and at what dilution?
bs-3157R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-GRB10 (Tyr67) Polyclonal Antibody (bs-3157R) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Horse, Rabbit.
How should bs-3157R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-GRB10 (Tyr67) Polyclonal Antibody (bs-3157R)?
KLH conjugated synthetic phosphopeptide derived from human GRB10 around the phosphorylation site of Tyr67 [SL(p-Y)SA]