Phospho-FRA1 (Ser265) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3158R-Cy5 |
| Product Name | Phospho-FRA1 (Ser265) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to Ser267 in Mouse and Ser269 in Rat. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human FRA1 around the phosphorylation site of Ser265 |
| Modification Site | Ser265 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8061 |
| Swiss Prot | P15407 |
| Subcellular location | Nucleus |
| Synonyms | FRA; FRA1; fra-1; Fos-related antigen 1; FOSL1 |
| Background | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-FRA1 (Ser265) Polyclonal Antibody, Cy5 Conjugated (bs-3158R-Cy5) validated for, and at what dilution?
bs-3158R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-FRA1 (Ser265) Polyclonal Antibody, Cy5 Conjugated (bs-3158R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.
How should bs-3158R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-FRA1 (Ser265) Polyclonal Antibody, Cy5 Conjugated (bs-3158R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human FRA1 around the phosphorylation site of Ser265
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3158R-Cy5)?
Cy5 on bs-3158R-Cy5: excitation / emission 625,650nm/670nm.