phospho-Histone H1.4 (Thr18) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3171R-Cy5-100uL |
| Product Name | phospho-Histone H1.4 (Thr18) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is found in Rat H1C as well, but not Human or Mouse H1C. This phosphorylation site is homologous to Thr18 in Rat H1C/H1D and Mouse H1D. Due to the highly conserved nature of these histones, this antibody may react with these members when phosphorylated at this site. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18 |
| Modification Site | Thr18 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3008 |
| Swiss Prot | P10412 |
| Subcellular location | Nucleus |
| Synonyms | H1E; H1.4; H1F4; H1s-4; dJ221C16.5; Histone H1.4; Histone H1b; Histone H1s-4; HIST1H1E |
| Background | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Histone H1.4 (Thr18) Polyclonal Antibody, Cy5 Conjugated (bs-3171R-Cy5) validated for, and at what dilution?
bs-3171R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Histone H1.4 (Thr18) Polyclonal Antibody, Cy5 Conjugated (bs-3171R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-3171R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Histone H1.4 (Thr18) Polyclonal Antibody, Cy5 Conjugated (bs-3171R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3171R-Cy5)?
Cy5 on bs-3171R-Cy5: excitation / emission 625,650nm/670nm.