Phospho-HDAC3 (Ser424) Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3174R-PE-Cy5-100uL |
| Product Name | Phospho-HDAC3 (Ser424) Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HDAC3 around the phosphorylation site of Ser424 |
| Modification Site | Ser424 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8841 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HDAC3 phospho S424; HDAC3 phospho Ser424; p-HDAC3 Ser424; RPD3 2; RPD3-2; HD 3; HD3; HDAC 3; Histone deacetylase 3 HD3 RPD3-2; histone deacetylase 3; RPD 3; RPD3; SMAP 45; SMAP-45; SMAP45; HDAC3_HUMAN. |
| Background | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-HDAC3 (Ser424) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3174R-PE-Cy5) validated for, and at what dilution?
bs-3174R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-HDAC3 (Ser424) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3174R-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-3174R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-HDAC3 (Ser424) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3174R-PE-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human HDAC3 around the phosphorylation site of Ser424
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-3174R-PE-Cy5)?
PE-Cy5 on bs-3174R-PE-Cy5: excitation / emission 488nm/670nm.