phospho-GAB2 (Ser159) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3177R-Cy5.5-100uL |
| Product Name | phospho-GAB2 (Ser159) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human GAB2 around the phosphorylation site of Ser159 |
| Modification Site | Ser159 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9846 |
| Swiss Prot | Q9UQC2 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | GRB2-associated-binding protein 2; GRB2-associated binder 2; Growth factor receptor bound protein 2-associated protein 2; pp100; GAB2; KIAA0571 |
| Background | Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-GAB2 (Ser159) Polyclonal Antibody, Cy5.5 Conjugated (BS-3177R-CY5-5) validated for, and at what dilution?
BS-3177R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-GAB2 (Ser159) Polyclonal Antibody, Cy5.5 Conjugated (BS-3177R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Pig, Horse, Chicken, Rabbit.
How should BS-3177R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-GAB2 (Ser159) Polyclonal Antibody, Cy5.5 Conjugated (BS-3177R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human GAB2 around the phosphorylation site of Ser159
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-3177R-CY5-5)?
Cy5.5 on BS-3177R-CY5-5: excitation / emission 675nm/694nm.