phospho-GAB2 (Tyr452) Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3178R-APC-Cy7-100uL |
| Product Name | phospho-GAB2 (Tyr452) Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human GAB2 around the phosphorylation site of Tyr452 |
| Modification Site | Tyr452 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9846 |
| Swiss Prot | Q9UQC2 |
| Subcellular location | Cytoplasm |
| Synonyms | GRB2-associated-binding protein 2; GRB2-associated binder 2; Growth factor receptor bound protein 2-associated protein 2; pp1; GAB2; KIAA571 |
| Background | Adapter protein which acts downstream of several membrane receptors including cytokine, antigen, hormone, cell matrix and growth factor receptors to regulate multiple signaling pathways. Regulates osteoclast differentiation mediating the TNFRSF11A/RANK signaling. In allergic response, it plays a role in mast cells activation and degranulation through PI-3-kinase regulation. Also involved in the regulation of cell proliferation and hematopoiesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-GAB2 (Tyr452) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3178R-APC-Cy7) validated for, and at what dilution?
bs-3178R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3178R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-GAB2 (Tyr452) Polyclonal Antibody, APC-Cy7 Conjugated (bs-3178R-APC-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human GAB2 around the phosphorylation site of Tyr452
What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-3178R-APC-Cy7)?
APC-Cy7 on bs-3178R-APC-Cy7: excitation / emission 650nm/780nm.