Phospho-Histone H3 (Ser28) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3187R-Cy3 |
| Product Name | Phospho-Histone H3 (Ser28) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
|
| Specificity | This phosphorylation site is homologous across the listed species. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig, Rabbit, Fruit Fly |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser29 |
| Modification Site | Ser28 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8290 |
| Swiss Prot | Q16695 |
| Synonyms | H3t; H3.4; H3/g; H3FT; Histone H3.1t; H3/t; HIST3H3 |
| Background | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-Histone H3 (Ser28) Polyclonal Antibody, Cy3 Conjugated (bs-3187R-Cy3) validated for, and at what dilution?
bs-3187R-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Histone H3 (Ser28) Polyclonal Antibody, Cy3 Conjugated (bs-3187R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig, Rabbit, Fruit Fly.
How should bs-3187R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Histone H3 (Ser28) Polyclonal Antibody, Cy3 Conjugated (bs-3187R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser29
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3187R-Cy3)?
Cy3 on bs-3187R-Cy3: excitation / emission 512,550nm/570,615nm.