Phospho-IKB beta (Ser23) Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3191R-BF750-100uL |
| Product Name | Phospho-IKB beta (Ser23) Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human NFKBIB around the phosphorylation site of Ser23 |
| Modification Site | Ser23 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4793 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | IKB beta Ser23; IKB beta S23; p-IKB beta Ser23; p-IKB beta S23; NFKBIBSer23; NFKBIBphospho S23; IkB-betaSer22; IkB beta; NF-kappa-B inhibitor beta; IKB beta; NF-kappa-BIB; TRIP-9; I-kappa-B-beta; IkappaBbeta; IkB-beta; IkB-B; Thyroid receptor-interacting protein 9; TR-interacting protein 9; NFKBIB; IKBB. |
| Background | The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-IKB beta (Ser23) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-3191R-BF750) validated for, and at what dilution?
bs-3191R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-3191R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-IKB beta (Ser23) Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-3191R-BF750)?
KLH conjugated synthetic phosphopeptide derived from human NFKBIB around the phosphorylation site of Ser23
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-3191R-BF750)?
AbBy Fluor-750 on bs-3191R-BF750: excitation / emission 749nm/775nm.