Phospho-IRAK1 (Ser376) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3192R-Cy5 |
| Product Name | Phospho-IRAK1 (Ser376) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse IRAK1 around the phosphorylation site of Ser376 [QS(p-S)TV] |
| Modification Site | Ser376 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 16179 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | IRAK1 Ser376; IRAK1 S376; IRAK1 p-Ser376; Il1rak; Il1rak; Interleukin 1 receptor associated kinase 1; Interleukin 1 receptor associated kinase 1; Interleukin 1 receptor associated kinase 2; Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; IRAK1; IRAK1; IRAK1_HUMAN; IRAK2; IRAK2; mPLK; mPLK; Pelle; Pelle; Pelle homolog; Pelle-like protein kinase; Plpk. |
| Background | IRAK or Interleukin-1 Receptor-associated Kinase 1, is one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This protein is partially responsible for IL1-induced upregulation of the ubiquitous transcription factor NF-kappa B. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-IRAK1 (Ser376) Polyclonal Antibody, Cy5 Conjugated (bs-3192R-Cy5) validated for, and at what dilution?
bs-3192R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-IRAK1 (Ser376) Polyclonal Antibody, Cy5 Conjugated (bs-3192R-Cy5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Rabbit.
How should bs-3192R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-IRAK1 (Ser376) Polyclonal Antibody, Cy5 Conjugated (bs-3192R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from mouse IRAK1 around the phosphorylation site of Ser376 [QS(p-S)TV]
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3192R-Cy5)?
Cy5 on bs-3192R-Cy5: excitation / emission 625,650nm/670nm.