Phospho-IRAK1 (Thr209) Polyclonal Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bs-3193R-100uL |
| Product Name | Phospho-IRAK1 (Thr209) Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
FLOW-CYT
ICC/IF
|
| Specificity | This phosphorylation site is homologous to Thr209 in Mouse and Rat. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr209 [RG(p-T)HN] |
| Modification Site | Thr209 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3654 |
| Swiss Prot | P51617 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | IRAK; pelle; Interleukin-1 receptor-associated kinase 1; IRAK-1; IRAK1 |
| Background | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | FCM1:20-100 |
| IHC-F | IHC-P1:200-400 |
| IF | ICC1:50-200 |
| Flow-Cyt | |
| ICC/IF | |
Frequently asked questions
Which applications is Phospho-IRAK1 (Thr209) Polyclonal Antibody (bs-3193R) validated for, and at what dilution?
bs-3193R is validated for WB (1:300-5000); FCM (1:20-100); IHC-P (1:200-400); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-IRAK1 (Thr209) Polyclonal Antibody (bs-3193R) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-3193R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-IRAK1 (Thr209) Polyclonal Antibody (bs-3193R)?
KLH conjugated synthetic phosphopeptide derived from human IRAK1 around the phosphorylation site of Thr209 [RG(p-T)HN]