phospho-HDAC6 (Ser22) Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3215R-Biotin |
| Product Name | phospho-HDAC6 (Ser22) Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HDAC6 around the phosphorylation site of Ser22 |
| Modification Site | Ser22 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 10013 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HDAC6 phospho S22; HDAC6 phospho Ser22; p-HDAC6 Ser22; HD 6; HD6; HDAC 6; Histone deacetylase 6; HD6; Histone deacetylase 6; JM 21; JM21; KIAA0901; FLJ16239; HDAC6_HUMAN. |
| Background | HDAC6 is a member of the class II mammalian histone deacetylases. Human HDAC6 is composed of 1215 amino acid residues. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is phospho-HDAC6 (Ser22) Polyclonal Antibody, Biotin Conjugated (bs-3215R-Biotin) validated for, and at what dilution?
bs-3215R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-HDAC6 (Ser22) Polyclonal Antibody, Biotin Conjugated (bs-3215R-Biotin) react with?
Tested reactivity: Human.
How should bs-3215R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-HDAC6 (Ser22) Polyclonal Antibody, Biotin Conjugated (bs-3215R-Biotin)?
KLH conjugated synthetic phosphopeptide derived from human HDAC6 around the phosphorylation site of Ser22