phospho-HDAC6 (Ser22) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3215R-Cy5 |
| Product Name | phospho-HDAC6 (Ser22) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HDAC6 around the phosphorylation site of Ser22 |
| Modification Site | Ser22 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10013 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | HDAC6 phospho S22; HDAC6 phospho Ser22; p-HDAC6 Ser22; HD 6; HD6; HDAC 6; Histone deacetylase 6; HD6; Histone deacetylase 6; JM 21; JM21; KIAA0901; FLJ16239; HDAC6_HUMAN. |
| Background | HDAC6 is a member of the class II mammalian histone deacetylases. Human HDAC6 is composed of 1215 amino acid residues. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-HDAC6 (Ser22) Polyclonal Antibody, Cy5 Conjugated (bs-3215R-Cy5) validated for, and at what dilution?
bs-3215R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-HDAC6 (Ser22) Polyclonal Antibody, Cy5 Conjugated (bs-3215R-Cy5) react with?
Tested reactivity: Human.
How should bs-3215R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-HDAC6 (Ser22) Polyclonal Antibody, Cy5 Conjugated (bs-3215R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human HDAC6 around the phosphorylation site of Ser22
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3215R-Cy5)?
Cy5 on bs-3215R-Cy5: excitation / emission 625,650nm/670nm.