phospho-HDAC7 (Ser358) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3216R-Cy5 |
| Product Name | phospho-HDAC7 (Ser358) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HDAC7 around the phosphorylation site of Ser358 |
| Modification Site | Ser358 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | HDAC7 phospho S358; HDAC7 phospho Ser358; p-HDAC7 Ser358; HD 7a; HD7a; HDAC 7; HDAC 7A; HDAC7; HDAC7A; Histone deacetylase 7; Histone deacetylase 7A; DKFZP586J0917; OTTHUMP00000202813; OTTHUMP00000202814; FLJ99588; HDAC7_HUMAN. |
| Background | HDAC7 is a member of the class II mammalian histone deacetylases, which plays an important role in modulating the eukaryotic chromatin structure. Human HDAC7 is composed of 912 amino acid residues. Although HDAC7 is localized mostly to the cell nucleus, it is also found in the cytoplasm, suggesting nucleo-cytoplasmic shuttling. The histone deacetylase activity of HDAC7 maps to a carboxy-terminal domain and is dependent on interaction with class I HDACs in the nucleus. It is an active component of different transcriptional corepressor complexes that can be recruited to specific promoter regions via interactions with a growing number of sequence specific transcriptional factors. HDAC7 catalyzes removal of acetyl-groups from acetyl-lysines of histones and promotes compaction of chromatin in these regions, leading to the inhibition of gene transcription. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-HDAC7 (Ser358) Polyclonal Antibody, Cy5 Conjugated (bs-3216R-Cy5) validated for, and at what dilution?
bs-3216R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-HDAC7 (Ser358) Polyclonal Antibody, Cy5 Conjugated (bs-3216R-Cy5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-3216R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-HDAC7 (Ser358) Polyclonal Antibody, Cy5 Conjugated (bs-3216R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human HDAC7 around the phosphorylation site of Ser358
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3216R-Cy5)?
Cy5 on bs-3216R-Cy5: excitation / emission 625,650nm/670nm.