Phospho-HP1 gamma (Ser93) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-3221R-Cy5.5-100uL |
| Product Name | Phospho-HP1 gamma (Ser93) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human HP1 gamma around the phosphorylation site of Ser83 |
| Modification Site | Ser93 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11335 |
| Subcellular location | Nucleus |
| Synonyms | HP1 gamma phospho S93; HP1 gamma phospho Ser93; p-HP1 gamma Ser93; CBX 3; CBX3; Chromobox homolog 3; Chromobox protein homolog 3; HECH; Heterochromatin like protein 1; Heterochromatin protein 1 homolog gamma; Heterochromatin protein HP1 gamma; HP1 gamma homolog; HP1Hs gamma ; Modier 2; protein HP1-GAMMA; HP1g; M32; HP1 gamma; MGC118084; CBX3_HUMAN; Modier 2 protein. |
| Background | HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-HP1 gamma (Ser93) Polyclonal Antibody, Cy5.5 Conjugated (BS-3221R-CY5-5) validated for, and at what dilution?
BS-3221R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-3221R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-HP1 gamma (Ser93) Polyclonal Antibody, Cy5.5 Conjugated (BS-3221R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human HP1 gamma around the phosphorylation site of Ser83
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-3221R-CY5-5)?
Cy5.5 on BS-3221R-CY5-5: excitation / emission 675nm/694nm.