Phospho-HSL (Ser863) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3223R-Cy5 |
| Product Name | Phospho-HSL (Ser863) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from rat HSL around the phosphorylation site of Ser863 |
| Modification Site | Ser863 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 25330 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Hormone sensitive lipase; Hormone sensitive lipase testicular isoform; HSL; LHS; Lipase hormone sensitive; LIPE. |
| Background | HSL/LIPE is found in adipose tissue and heart, where it primarily hydrolyzes stored triglycerides to free fatty acids. It is also found in steroidogenic tissues, where it principally converts cholesteryl esters to free cholesterol for steroid hormone production. There are two named isoforms. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-HSL (Ser863) Polyclonal Antibody, Cy5 Conjugated (bs-3223R-Cy5) validated for, and at what dilution?
bs-3223R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-HSL (Ser863) Polyclonal Antibody, Cy5 Conjugated (bs-3223R-Cy5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-3223R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-HSL (Ser863) Polyclonal Antibody, Cy5 Conjugated (bs-3223R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from rat HSL around the phosphorylation site of Ser863
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3223R-Cy5)?
Cy5 on bs-3223R-Cy5: excitation / emission 625,650nm/670nm.