Phospho-Jak1 (Tyr1034 + Tyr1035) Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3238R-PE-Cy5.5-100uL |
| Product Name | Phospho-Jak1 (Tyr1034 + Tyr1035) Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Jak1 around the phosphorylation site of Tyr1034 + Tyr1035 |
| Modification Site | Tyr1034 + Tyr1035 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3716 |
| Swiss Prot | P23458 |
| Subcellular location | Cell membrane |
| Synonyms | Tyrosine-protein kinase JAK1; Janus kinase 1; JAK-1; JAK1; JAK1A; JAK1B |
| Background | Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-Jak1 (Tyr1034 + Tyr1035) Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-3238R-PE-CY5-5) validated for, and at what dilution?
BS-3238R-PE-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Jak1 (Tyr1034 + Tyr1035) Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-3238R-PE-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig.
How should BS-3238R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Jak1 (Tyr1034 + Tyr1035) Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-3238R-PE-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human Jak1 around the phosphorylation site of Tyr1034 + Tyr1035
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-3238R-PE-CY5-5)?
PE-Cy5.5 on BS-3238R-PE-CY5-5: excitation / emission 488nm/694nm.