Phospho-Cytokeratin 17(Ser44) Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3239R-PE-100uL |
| Product Name | Phospho-Cytokeratin 17(Ser44) Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CK 17 around the phosphorylation site of Ser44 |
| Modification Site | Ser44 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3872 |
| Subcellular location | Cytoplasm |
| Synonyms | CK17 phospho S44; CK17 phospho Ser44; p-CK17 Ser44; 39.1; CK 17; Cytokeratin17; K17; Keratin 17 antibody Keratin type I cytoskeletal 17; keratin, type I cytoskeletal 17; Keratin17; KRT 17; KRT17; KRT17 protein; PC; PC2; PCHC1; K1C17_HUMAN. |
| Background | The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is Phospho-Cytokeratin 17(Ser44) Polyclonal Antibody, PE Conjugated (bs-3239R-PE) validated for, and at what dilution?
bs-3239R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Cytokeratin 17(Ser44) Polyclonal Antibody, PE Conjugated (bs-3239R-PE) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-3239R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Cytokeratin 17(Ser44) Polyclonal Antibody, PE Conjugated (bs-3239R-PE)?
KLH conjugated synthetic phosphopeptide derived from human CK 17 around the phosphorylation site of Ser44
What are the excitation and emission wavelengths of the PE conjugate (bs-3239R-PE)?
PE on bs-3239R-PE: excitation / emission 496,564nm/578nm.