phospho-LKB1 (Ser334) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-3248R-Cy3 |
| Product Name | phospho-LKB1 (Ser334) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of Ser334 [WR(p-S)MT] |
| Modification Site | Ser334 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6794 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | STK11phospho S334; LKB 1; LKB1; NY REN 19 antigen; Peutz Jeghers syndrome; PJS; Serine/Threonine Kinase 11; Serine/threonine protein kinase 11; Serine/threonine protein kinase LKB1; STK 11; STK11. |
| Background | This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-LKB1 (Ser334) Polyclonal Antibody, Cy3 Conjugated (bs-3248R-Cy3) validated for, and at what dilution?
bs-3248R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-LKB1 (Ser334) Polyclonal Antibody, Cy3 Conjugated (bs-3248R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-3248R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-LKB1 (Ser334) Polyclonal Antibody, Cy3 Conjugated (bs-3248R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human LKB1 around the phosphorylation site of Ser334 [WR(p-S)MT]
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3248R-Cy3)?
Cy3 on bs-3248R-Cy3: excitation / emission 512,550nm/570,615nm.