Phospho-ALOX5 (Ser663) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3252R-Cy5.5-100uL |
| Product Name | Phospho-ALOX5 (Ser663) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phosphorylation site is homologous to that of Ser664 in Mouse and Human. |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from rat 5-Lipoxygenase around the phosphorylation site of Ser663 |
| Modification Site | Ser663 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 240 |
| Swiss Prot | P09917 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | 5-LO; 5LPG; LOG5; 5-LOX; Arachidonate 5-lipoxygenase; 5-lipoxygenase; ALOX5 |
| Background | Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-ALOX5 (Ser663) Polyclonal Antibody, Cy5.5 Conjugated (BS-3252R-CY5-5) validated for, and at what dilution?
BS-3252R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-ALOX5 (Ser663) Polyclonal Antibody, Cy5.5 Conjugated (BS-3252R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Rabbit.
How should BS-3252R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-ALOX5 (Ser663) Polyclonal Antibody, Cy5.5 Conjugated (BS-3252R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from rat 5-Lipoxygenase around the phosphorylation site of Ser663
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-3252R-CY5-5)?
Cy5.5 on BS-3252R-CY5-5: excitation / emission 675nm/694nm.