Phospho-Lyn (Tyr507) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3256R-Cy5.5-100uL |
| Product Name | Phospho-Lyn (Tyr507) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Chicken, Cat, Rabbit, Zebrafish, GuineaPig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Lyn around the phosphorylation site of Tyr508 |
| Modification Site | Tyr508 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4067 |
| Synonyms | Lyn phospho Y508; Lyn phospho Tyr508; p-Lyn Tyr508; Hck 2; JTK 8; JTK8; ONCOGENE LYN; Tyrosine protein kinase LYN; V yes 1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral v yes 1 related oncogene homolog; AA407514; EC 2.7.10.2; FLJ26625; LYN_HUMAN. |
| Background | Lyn (also known as p53/56 Lyn) is a membrane associated protein tyrosine kinase (PTK) mostly expressed in hemopoietic cells which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis. A member of the Src family of PTKs, there are two known isoforms for Lyn which plays an indispensable role in the Fc epsilon RI (Fcer1) and the B cell IgM receptor signaling pathway and is essential for Syk activation and Lat phosphorylation after Fcer1 aggregation and can also phosphorylate Tec on multiple residues. Lyn can also be regulated by IL2 and IL3. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-Lyn (Tyr507) Polyclonal Antibody, Cy5.5 Conjugated (BS-3256R-CY5-5) validated for, and at what dilution?
BS-3256R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Lyn (Tyr507) Polyclonal Antibody, Cy5.5 Conjugated (BS-3256R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, Cat, Rabbit, Zebrafish, GuineaPig.
How should BS-3256R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Lyn (Tyr507) Polyclonal Antibody, Cy5.5 Conjugated (BS-3256R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human Lyn around the phosphorylation site of Tyr508
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-3256R-CY5-5)?
Cy5.5 on BS-3256R-CY5-5: excitation / emission 675nm/694nm.