Phospho-Mcl1 (Ser159 + Thr163) Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3265R-PE-Cy5-100uL |
| Product Name | Phospho-Mcl1 (Ser159 + Thr163) Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FCM
|
| Specificity | These phosphorylation sites are homologous to Ser140 + Thr144 in Mouse and Ser139 + Thr143 in Rat. |
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Horse |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Mcl 1 around the phosphorylation site of Ser159/Thr163 |
| Modification Site | Ser159 + Thr163 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4170 |
| Swiss Prot | Q07820 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; MCL1 |
| Background | Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Phospho-Mcl1 (Ser159 + Thr163) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3265R-PE-Cy5) validated for, and at what dilution?
bs-3265R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-Mcl1 (Ser159 + Thr163) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3265R-PE-Cy5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Horse.
How should bs-3265R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-Mcl1 (Ser159 + Thr163) Polyclonal Antibody, PE-Cy5 Conjugated (bs-3265R-PE-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human Mcl 1 around the phosphorylation site of Ser159/Thr163
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-3265R-PE-Cy5)?
PE-Cy5 on bs-3265R-PE-Cy5: excitation / emission 488nm/670nm.