Phospho-MEK1/2 (Ser218 + Ser222) Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3270R-BF350 |
| Product Name | Phospho-MEK1/2 (Ser218 + Ser222) Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
|
| Specificity | These phosphorylation sites are homologous to Ser222 + Ser226 in MEK2 and are the same across the listed species. |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Ser218/222 |
| Modification Site | Ser218 + Ser222 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 56045605 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | MAP2K1phospho S218/S222; Dual specicity mitogen activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP kinase/Erk kinase 1; MAP/Erk kinase 1; Map2K1; MAPK/ERK kinase 1; MAPKK 1; MAPKK1; MEK 1; MEKK1; Mitogen activated protein kinase kinase 1; MKK 1; MKK1; PRKMK 1; PRKMK1;Protein kinase mitogen activated kinase 1 MAP kinase kinase 1; Protein kinase mitogen activated kinase 1; Protein kinase mitogen activated, kinase 1. |
| Background | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-MEK1/2 (Ser218 + Ser222) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-3270R-BF350) validated for, and at what dilution?
bs-3270R-BF350 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-MEK1/2 (Ser218 + Ser222) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-3270R-BF350) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Chicken, Rabbit.
How should bs-3270R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-MEK1/2 (Ser218 + Ser222) Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-3270R-BF350)?
KLH conjugated synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Ser218/222
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-3270R-BF350)?
AbBy Fluor-350 on bs-3270R-BF350: excitation / emission 346nm/442nm.