Phospho-MEK6 (Ser207) Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3275R-Cy3 |
| Product Name | Phospho-MEK6 (Ser207) Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MEK6 around the phosphorylation site of Ser207 |
| Modification Site | Ser207 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5608 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MAP kinase kinase 6; MAPK/ERK kinase 6; MAPKK6; MEK6; Mitogen Activated Protein Kinase Kinase 6; MKK 6; MKK6; PRKMK6; SAPKK3; Dual specicity mitogen activated protein kinase kinase 6; MKK 6; Dual specicity mitogen activated protein kinase kinase 6; EC 2.7.12.2; MP2K6_HUMAN. |
| Background | Mitogen activated protein kinase kinase 6 (MEK6 or MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MEK6, closely related to MEK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38, thus activating it, in response to inflammatory cytokines and environmental stress.As an essential component of p38 MAP kinase mediated signal transduction pathway, this protein is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-MEK6 (Ser207) Polyclonal Antibody, Cy3 Conjugated (bs-3275R-Cy3) validated for, and at what dilution?
bs-3275R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-MEK6 (Ser207) Polyclonal Antibody, Cy3 Conjugated (bs-3275R-Cy3) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-3275R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-MEK6 (Ser207) Polyclonal Antibody, Cy3 Conjugated (bs-3275R-Cy3)?
KLH conjugated synthetic phosphopeptide derived from human MEK6 around the phosphorylation site of Ser207
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-3275R-Cy3)?
Cy3 on bs-3275R-Cy3: excitation / emission 512,550nm/570,615nm.