Phospho-MEK7 (Ser271 + Thr275) Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3277R-PE |
| Product Name | Phospho-MEK7 (Ser271 + Thr275) Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MKK7 around the phosphorylation site of Ser271/Thr275 |
| Modification Site | Ser271 + Thr275 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5609 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MEK7 phospho S277 + T275; MEK7 phospho Ser277 + Thr275; p-MEK7 S277 + T275; p-MEK7 Ser277 + Thr275; c-Jun N-terminal kinase kinase 2; Dual specicity mitogen activated protein kinase kinase 7; Dual specicity mitogen-activated protein kinase kinase 7; JNK activating kinase 2; JNK kinase 2; JNK-activating kinase 2; Jnkk 2; Jnkk-2; Jnkk2; MAP kinase kinase 7; MAP2K7; MAPK/ERK kinase 7; MAPKK 7; MAPKK-7; MAPKK7; MEK 7; Mitogen Activated Protein Kinase kinase 7; MKK 7; MKK-7; MKK7; MP2K7_HUMAN; PRKMK 7; PRKMK-7; PRKMK7; Sek 2; Sek-2; Sek2. |
| Background | The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found, but only one transcript variant has been supported and defined. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is Phospho-MEK7 (Ser271 + Thr275) Polyclonal Antibody, PE Conjugated (bs-3277R-PE) validated for, and at what dilution?
bs-3277R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-MEK7 (Ser271 + Thr275) Polyclonal Antibody, PE Conjugated (bs-3277R-PE) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig.
How should bs-3277R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-MEK7 (Ser271 + Thr275) Polyclonal Antibody, PE Conjugated (bs-3277R-PE)?
KLH conjugated synthetic phosphopeptide derived from human MKK7 around the phosphorylation site of Ser271/Thr275
What are the excitation and emission wavelengths of the PE conjugate (bs-3277R-PE)?
PE on bs-3277R-PE: excitation / emission 496,564nm/578nm.