Phospho-MKP1 (Ser359) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3278R-Cy5 |
| Product Name | Phospho-MKP1 (Ser359) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MKP1 around the phosphorylation site of Ser359 |
| Modification Site | Ser359 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1843 |
| Subcellular location | Nucleus |
| Synonyms | Mitogen activated protein kinase phosphatase 1; CL 100; CL100; Dual Specicity Phosphatase 1; Dual specicity protein phosphatase 1; Dual specicity protein phosphatase hVH1; DUSP 1; EC 3.1.3.16; EC 3.1.3.48; HVH 1; HVH1; MAP kinase phosphatase 1; MKP 1; MKP1; MKP-1; Protein tyrosine phosphatase CL100; PTPN 10; PTPN10; Serine/threonine specic protein phosphatase; VH 1; VH1; 3ch134; Mkp1; Ptpn16. |
| Background | The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-MKP1 (Ser359) Polyclonal Antibody, Cy5 Conjugated (bs-3278R-Cy5) validated for, and at what dilution?
bs-3278R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-MKP1 (Ser359) Polyclonal Antibody, Cy5 Conjugated (bs-3278R-Cy5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Chicken, Rabbit.
How should bs-3278R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-MKP1 (Ser359) Polyclonal Antibody, Cy5 Conjugated (bs-3278R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human MKP1 around the phosphorylation site of Ser359
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-3278R-Cy5)?
Cy5 on bs-3278R-Cy5: excitation / emission 625,650nm/670nm.