Phospho-MSK1 (Ser376) Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-3283R-Cy7-100uL |
| Product Name | Phospho-MSK1 (Ser376) Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Ser376 [GY(p-S)FV] |
| Modification Site | Ser376 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9252 |
| Swiss Prot | O75582 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MSK1 Phospho Ser376; 90 kDa ribosomal protein S6 kinase 5; EC 2.7.11.1; KS6A5_HUMAN; MGC1911; Mitogen and stress activated protein kinase 1; MSPK1; Nuclear Mitogen And Stress Activated Protein Kinase 1; Nuclear mitogen- and stress-activated protein kinase 1; Ribosomal protein S6 kinase 90kD polypeptide 5; Ribosomal protein S6 kinase 90kDa; Ribosomal protein S6 kinase 90kDa polypeptide 5; Ribosomal Protein S6 Kinase Alpha 5; Ribosomal protein S6 kinase alpha-5; RLPK; RPS6KA5; RSK Like Protein Kinase; RSK-like protein kinase; RSKL; S6K alpha 5; S6K-alpha-5. |
| Background | MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG14 in response to mitogen and stress. Similar to RSK, MSK1 contains two kinase domains (N term and a C term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-MSK1 (Ser376) Polyclonal Antibody, Cy7 Conjugated (bs-3283R-Cy7) validated for, and at what dilution?
bs-3283R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-MSK1 (Ser376) Polyclonal Antibody, Cy7 Conjugated (bs-3283R-Cy7) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Rabbit.
How should bs-3283R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-MSK1 (Ser376) Polyclonal Antibody, Cy7 Conjugated (bs-3283R-Cy7)?
KLH conjugated synthetic phosphopeptide derived from human MSK1 around the phosphorylation site of Ser376 [GY(p-S)FV]
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-3283R-Cy7)?
Cy7 on bs-3283R-Cy7: excitation / emission 743nm/767nm.